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Presence of PCR product indicated by methylated (lane M) or unmethylated (lane U) alleles. MSP analysis of the RASSF1a, APC, MTSS1,CCND1, RB1, P16, CASP8 and FHIT genes in mock control and siDNMT3b-transfected cells. Results of MSP performed in (A) SMMC-7721 and (B) BEL-7402 cells. U, amplified using unmethylated primer; M, amplified using methylated primer; siRNA, small interfering RNA; MSP, methylation-specific polymerase chain reaction. siRNA, small interfering RNA.
