Table I.
First antibody dilution, pre-treatment and sources.
| First antibody | Clone no. | Dilution | Pre-treatment | Sourcea |
|---|---|---|---|---|
| L1 | UJ127 | 1:100 | High pressure retrieval in citric acid buffer (pH 6.0) | NeoMarkers |
| CD24 | SN3b | 1:75 | High pressure retrieval in citric acid buffer (pH 6.0) | NeoMarkers |
| Integrin β1 | 4B7R | 1:150 | - | Santa Cruz Biotechnology, Inc. |
| Ki-67 | MIB-1 | 1:200 | High pressure retrieval in citric acid buffer (pH 6.0) | Dako |
a
Neomarkers, Thermo Fisher Scientific, Waltham, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA; Dako, Glostrup, Denmark. -, the section was not treated; CD24, cluster of differentiation 24.