Table 1. Dauer formation of transgenic lines, CAN ablated animals, and dau-1 mutants in response to RS2333 and RS5134 pheromone.
| strain or line | chol. a | RS2333 pheromone | RS5134 pheromone | |||
|---|---|---|---|---|---|---|
| dauer (%) b | p value c | dauer (%) b | p value c | |||
| a | RS2333 (CA) | + | 11 | 5 | ||
| b | RS5134 (OH) | + | 68 | 39 | ||
| c1 | RS5134;Ex[dau-1.1 CA] RFP+ | + | 0 | <0.0001 b | 0 | <0.0001 b |
| c2 | RS5134;Ex[dau-1.1 CA] RFP- | + | 62 | 0.6001 b | 29 | 0.4710 b |
| d1 | RS2333;Ex[dau-1.1 CA] line1 RFP+ | + | 0 | 0.0009 a | 0 | 0.0827 a |
| d2 | RS2333;Ex[dau-1.1 CA] line1 RFP- | + | 11 | 1 a | 7 | 0.5883 a |
| e1 | RS2333;Ex[dau-1.1 CA] line2 RFP+ | + | 0 | 0.0101 a | 0 | 0.1871 a |
| e2 | RS2333;Ex[dau-1.1 CA] line2 RFP- | + | 6 | 0.7355 a | 7 | 0.6565 a |
| f1 | RS2333;Ex[dau-1.1 CA] line3 RFP+ | + | 0 | 0.0046 a | 0 | 0.0930 a |
| f2 | RS2333;Ex[dau-1.1 CA] line3 RFP- | + | 10 | 1 a | 6 | 1 a |
| g1 | RS2333;Ex[dau-1.1 OH] RFP+ | + | 0 | 0.0720 a | 0 | 0.3369 a |
| g2 | RS2333;Ex[dau-1.1 OH] RFP- | + | 12 | 1 a | 4 | 1 a |
| h1 | RS2333;Ex[egl-20::RFP] RFP+ | + | 10 | 1 a | 6 | 0.7455 a |
| h2 | RS2333;Ex[egl-20::RFP] RFP- | + | 9 | 0.7799 a | 7 | 0.4486 a |
| i | RS2333 (CA) | - | 22 | 0.0472 a | 14 | 0.0418 a |
| j | RS5134 (OH) | - | 87 | 0.0002 b | 53 | 0.0215 b |
| k1 | RS5134;Ex[dau-1.1 CA] RFP+ | - | 0 | 1 c1 | 0 | 1 c1 |
| k2 | RS5134;Ex[dau-1.1 CA] RFP- | - | 82 | 0.4170 c2 | 55 | 0.2082 c2 |
| l1 | RS2333;Ex[dau-1.1 CA] line1 RFP+ | - | 0 | 1 d1 | 0 | 1 d1 |
| l2 | RS2333;Ex[dau-1.1 CA] line1 RFP- | - | 16 | 1 d2 | 20 | 0.3650 d2 |
| m1 | RS2333;Ex[dau-1.1 CA] line2 RFP+ | - | 0 | 1 e1 | 0 | 1 e1 |
| m2 | RS2333;Ex[dau-1.1 CA] line2 RFP- | - | 20 | 0.3364 e2 | 10 | 1 e2 |
| n1 | RS2333;Ex[dau-1.1 CA] line3 RFP+ | - | 0 | 1 f1 | 0 | 1 f1 |
| n2 | RS2333;Ex[dau-1.1 CA] line3 RFP- | - | 19 | 1 f2 | 15 | 0.6026 f2 |
| o1 | RS2333;Ex[dau-1.1 OH] RFP+ | - | 0 | 1 g1 | 0 | 1 g1 |
| o2 | RS2333;Ex[dau-1.1 OH] RFP- | - | 22 | 0.3255 g2 | 13 | 0.3232 g2 |
| p1 | RS2333;Ex[egl-20::RFP] RFP+ | - | 21 | 0.1506 h1 | 13 | 0.2274 h1 |
| p2 | RS2333;Ex[egl-20::RFP] RFP- | - | 23 | 0.1176 h2 | 17 | 0.3320 h2 |
| q | RS2333 | - | 21 | 15 | ||
| r | RS2333 CAN- | - | 94 | <0.0001 q | 75 | <0.0001 q |
| s | RS5134 | - | 79 | 58 | ||
| t | RS5134 CAN- | - | 88 | 0.4149 s | 83 | 0.0156 s |
| u | RS2333;Ex[dau-1.1::RFP] | - | 0 | 0 | ||
| v | RS2333;Ex[dau-1.1::RFP] CAN- | - | 95 | <0.0001 u | 78 | <0.0001 u |
| w1 | dau-1.1(tu490) | - | 83 | <0.0001 q | 54 | 0.0001 q |
| 0.7953 s | 0.8405 s | |||||
| w2 | dau-1.1(tu491) | - | 79 | <0.0001 q | 59 | <0.0001 q |
| 1 s | 1 s | |||||
| w3 | dau-1.2(tu492) | - | 78 | <0.0001 q | 55 | <0.0001 q |
| 1 s | 1 s | |||||
| x1 | dau-1.1(tu490);dau-1.2(tu492) | - | 91 | 0.7150 r | 77 | 0.8153 r |
| 0.5536 w1 | 0.0196 w1 | |||||
| 0.2623 w2 | 0.0828 w2 | |||||
| 0.1714 w3 | 0.0327 w3 | |||||
| x2 | dau-1.1(tu490);dau-1.2(tu492)+DA | - | 0 | <0.0001 x1 | 0 | <0.0001 x1 |
| y | daf-12(tu389) | - | 0 | 0 | ||
| z | tu389;tu490;tu492 | - | 0 | <0.0001 x1 | 0 | <0.0001 x1 |
apresence (+) or absence (-) of cholesterol in NGM agar plates.
bmean dauer formation of three biological replicates.
c p value of Fisher's exact test.