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. 2015 Jun 19;5:10944. doi: 10.1038/srep10944

Figure 1. C2C12 electric pulse stimulation as a model to investigate muscle-liver crosstalk.

Figure 1

A: Experimental set-up, stimulation of FAO hepatocytes and primary rat hepatocytes with conditioned medium of C2C12 with and without Electric Pulse Stimulation (EPS) for 24 h. B: Representative Western blot analysis of phosphorylation levels of AMPK in C2C12 cells with and without EPS (left panel). Quantification of p-AMPK over AMPK ratio was performed on 3 individual experiments (right panel). C: Concentrations of IL-6, MCP-1 and KC in the conditioned medium of C2C12 cells with and without EPS measured by ELISA. Error bars represent SD values. ** = p < 0.01; *** = p < 0.001.