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. 2015 Jun 19;5:10944. doi: 10.1038/srep10944

Figure 3. A polar small molecule regulates the induction of Metallothionein expression by C2C12 CM.

Figure 3

Relative gene expression of Mt1 and Mt2 was measured by RT-PCR. A: FAO hepatocytes were treated with full CM, a protein fraction (>5 kDa) and a small molecule fraction (<5 kDa) for 16 hours. B: FAO hepatocytes were treated with full CM, a polar small molecule fraction and an apolar small molecule fraction for 16 hours. Error bars represent SD values of biological triplicates. *** = p < 0.001 compared to CM without EPS from the same fraction.