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. 2015 Jun 19;5:11064. doi: 10.1038/srep11064

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(A) Microbubbles observed by an inverted fluorescence microscope with a 100× objective lens (NA of 1.3). (B) Size distribution of microbubbles measured by digital image processing techniques. (C) An instantaneous velocity field of PBS solution with microbubbles in the vascular phantom at a flow rate of 10 mL/h. The corresponding ultrasound image is superimposed on the velocity field data. (D) Normalized velocity profile (V/V_max) along the normalized radial direction (r/R). The theoretical parabolic velocity profile is included for comparison. The flow rate of PBS solution is evaluated by Eq. (3).