FIG 7.

HeV G N-glycans protect HeV against neutralizing antibodies. Pseudotyped HeV/VSV-rLuc virions with incorporated WT HeV F and WT or mutant HeV G were neutralized with various concentrations of HeV G-specific rabbit polyclonal antiserum 837 prior to infecting Vero cells. Infected Vero cells were measured for relative light units (RLU) 18 to 24 h postinfection. Single (A) and multiple (B) HeV G N-glycan mutants were tested. Logarithms of the dilutions of 1 mg/ml serum stocks are shown. The average results ± SEM from at least six independent experiments with triplicate wells per experiment are shown. The 50% inhibitory concentration (IC₅₀) is represented by a dotted line.