TABLE 1.
Fusion indices for HeV G wild-type and N-glycan mutant glycoproteins and comparison to published NiV G fusion indicesa
| G env | HeV G |
NiV G (FI ± SEM)b | ||
|---|---|---|---|---|
| CSE | Fusion | FI ± SEM | ||
| WT | 100 | 100 | 1.00 | 1.00 |
| N-glycan mutants | ||||
| G1 | 90 | 18 | 0.20 ± 0.07 | 0.31 ± 0.06 |
| G2 | 58 | 0 | 0.00 ± 0.00 | 0.02 ± 0.03 |
| N275Q | 58 | 25 | 0.43 ± 0.14 | ND |
| G3** | 77 | 100 | 1.29 ± 0.04 | 0.86 ± 0.11 |
| G4** | 89 | 146 | 1.64 ± 0.08 | 1.11 ± 0.10 |
| G5 | 70 | 63 | 0.89 ± 0.09 | 0.67 ± 0.06 |
| G6** | 43 | 110 | 2.55 ± 0.21 | 6.38 ± 0.45 |
| G7** | 75 | 74 | 0.99 ± 0.13 | 1.60 ± 0.09 |
| G2G3 | 31 | 0 | 0.00 ± 0.00 | 0.10 ± 0.06 |
| G3G4 | 57 | 120 | 2.11 ± 0.25 | ND |
| G3G5 | 39 | 98 | 2.47 ± 0.47 | ND |
| G4G5 | 37 | 102 | 2.91 ± 0.26 | 2.50 ± 0.29 |
| G6G7** | 12 | 33 | 2.38 ± 0.13 | 1.46 ± 0.09 |
| G4G7* | 28 | 84 | 3.02 ± 0.35 | 4.13 ± 0.21 |
| G3G4G5** | 15 | 173 | 11.81 ± 1.09 | 4.49 ± 0.54 |
| G4G5G6 | 2 | 0 | — | 5.88 ± 0.41 |
| G4G6G7 | 1 | 1 | — | 6.71 ± 0.43 |
| G3G4G5G6 | 0 | 0 | — | ND |
a
Statistical analysis was done using the Student t test (two-sample assuming equal variances). Statistically significant differences between FIs (fusion indices [fusion/CSE ratios]) from both viruses are indicated by boldface and by asterisks as follows: **, P < 0.01; *, P < 0.05. —, sample for which a valid FI could not be determined due to no or minimal surface expression; ND, not determined; CSE, cell surface expression; WT, wild type G.
b
NiV G FIs were previously published by Biering et al. (14), with the exception of NiV G3G4G5, which was determined in this study.