FIG 3.

Codon optimization of v-FLIP gene sequence increases abundance of protein and RNA. (A) Western blot of proteins from 293 cells that were transfected with recombinant FLAG-tagged v-FLIP. Western blotting was done using an anti-FLAG monoclonal antibody. (B) Northern blot of increasing amounts of RNA (1 μg, 5 μg, and 10 μg of total RNA), denoted by the number of plus signs, is shown. v-FLIP RNA is detected with a biotin-labeled oligonucleotide probe complementary to the FLAG tag sequences of v-FLIP; neo transcripts are detected with a probe against the neo gene present on the vector encoding recombinant v-FLIP.