FIG 7.

Reduced stability of partially optimized (construct 7) RNA compared to completely optimized (HS) RNA. (A) RNA from 293 cells that had been transfected with construct 7 or HS v-FLIP was purified after treatment with actinomycin D for the indicated times. Northern blotting was done using labeled probe against the invariant 5′ FLAG tag. (B) Doxycycline-induced repression of transfected v-FLIP genes (construct 7 or HS) was instituted at 48 h posttransfection (here designated 0 h), and RNA was harvested at the indicated times thereafter and examined by Northern blotting against the invariant FLAG tag of v-FLIP.