FIG 8.

Cloning a stem-loop into the 5′ UTR and mutating the v-FLIP start codon does not enhance accumulation of v-FLIP mRNA. 293 cells were transfected with the indicated plasmid construct, and 48 h later, total RNA was prepared for Northern blotting. All constructs were tagged with a 5′ FLAG tag, and a probe complementary to this sequence was employed to detect the transcripts. Lanes 2 to 4, variants of the HS (codon-optimized) FLIP sequence; lanes 5 to 7, variants of the WT sequence. Abbreviations: SL, stem-loop sequence; ΔAUG, start codon deletion.