FIG 3.

GAS-dependent cytokine augmentation of the IFN response. (A and B) Huh-7 cells were transfected with GAS-linked EGFP reporter constructs. After 20 h of incubation, the cells were treated with 1 nM each selected cytokine cluster in the presence (A) or absence (B) of 1 nM IFN-γ. (C and D) Huh-7 cells were transfected with GAS-linked reporter constructs for 20 h and then were treated with increasing doses of IFN-γ in the absence or presence of 1 nM OSM (C) or BTC (D). CC, cell control. The data shown are mean (and SEM) fold increases in fluorescence activities (pixels) from three independent experiments; P < 0.0001 as determined by two-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.