FIG 2.

MV does not form syncytia in HAE. HAE were infected from the basolateral surface with MV-GFP. Three days later, GFP expression was documented (A), the cell surface was stained with wheat germ agglutinin (WGA) (B), and nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (C). A merged image is shown (D). MV N protein colocalizes with GFP in infectious centers. Three days postinfection with MV-GFP, HAE were stained with anti-N protein antibody. GFP expression (E) and N protein staining (F) were observed in almost every MV-infected cell with few exceptions (white arrow). Panels E and F each represent a digitally compressed z-stack; panel G is a merged image of a single z-slice from panels E and F. Bars, 50 μm.