FIG 3.

Mice challenged with rZH501 with a single mutation in the MP-12 M segment. Survival curves for outbred CD1 mice (n = 10 per group) challenged with a 1 × 10³-PFU dose (i.p.) of rZH501 with one mutation in the MP-12 M segment are shown. (A) Mutations U46C (I9T) and G69A (V17I) in the 78-kDa protein; (B) mutations U795C (Y259H) and G857A in Gn; (C) mutations A2259U (I747L) and A3564G (R1182G) in Gc; (D) mutations A3621G, A3644G, and A3660U in the 5′ UTR. P values obtained by the log-rank test (versus the results for parental strain rZH501) are shown (*, P < 0.05; **, P < 0.01). (E) Clinical signs of diseases in mice challenged with the U795C [Y259H (Gn)] or A3564G [R1182G (Gc)] mutant. Gray shading, period of disease in parental strain rZH501-infected mice. Anti-N IgG was not detectable in a surviving mouse challenged with the A3564G mutant (#). (F) Change in body weight (in percent) at 3 dpi compared to the body weight at 0 dpi. The bars represent the mean and standard error. P values obtained by the Mann-Whitney test (versus the results for RST-MP12-M) are shown (*, P < 0.05; **, P < 0.01). The dotted line indicates a 100% change in body weight. ns, not significant.