FIG 8.

Mice challenged with rMP-12 with M- and L-segment reversion mutations. (A) Survival curves for outbred CD1 mice (n = 10 per group) challenged with a 1 × 10³-PFU dose (i.p.) of rMP-12 with a reversion mutation(s): K1029R(L) only; H259Y (Gn) only; G1182R (Gc) only; H259Y (Gn) and G1182R(Gc); or H259Y (Gn), G1182R (Gc), and K1029R (L). Survival curves were not statistically significantly different by the log-rank test (versus the results for the K1029R mutant). (B) Clinical signs of disease in mice challenged with rMP-12 with a reversion mutation(s), i.e., H259Y (Gn) only; G1182R (Gc) only; H259Y (Gn) and G1182R (Gc); or H259Y (Gn), G1182R (Gc), and K1029R (L). Gray shading, period of disease in parental strain rZH501-infected mice. There were no detectable clinical signs of disease in mice infected with the K1029R (L) mutant (not shown). (C) Change in body weight (in percent) at 3 dpi compared to the body weight at 0 dpi. The Mann-Whitney test (versus the results for the K1029R mutant) was performed, yet no statistically significant differences were found. ns, not significant. The dotted line indicates a 100% change in body weight. The bars represent the mean and standard error.