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. 2015 May 6;89(14):7314–7328. doi: 10.1128/JVI.00603-15

FIG 3.

FIG 3

Viral growth in the absence of pUL47. (A) Single-step growth kinetics assays of wild-type TB40-BAC4, revertant TB-47rev, and mutant TB-47stop viruses were performed by infecting HFFs at an MOI of 3. Virus yields of the supernatants were determined at the indicated times. Virus yields at day 0 represent the input virus remaining after infection. Shown are the mean and standard deviation of results for four experiments. (B) Relative genome copies in the supernatants of TB40-BAC4, TB-47stop, and TB-47rev viruses at day 7 postinfection. Relative genomic copies were determined by real-time PCR and calculated from a standard curve. Relative genome copies of TB40-BAC4 were set as 100%. For the statistical analysis, the two-tailed Student t test was used (*, P = 0.0189; n.s., not significant. (C) The graph depicts the ratio between the relative genome copies and the virus yields in the supernatants of TB40-BAC4, TB-47stop, and TB-47rev viruses at day 7 postinfection (standard deviations are shown).