FIG 6.

Indirect immunofluorescence of HFFs infected with TB40-BAC4, TB-47stop, or TB-47rev virus at day 5 postinfection. (A) Intracellular distributions of viral proteins were compared by staining pUL48 (green) with our polyclonal serum and counterstaining with either anti-pp150 MAb or anti-pp28 MAb (red). White arrows indicate peripheral accumulations of pUL48 and pp150 in TB-47stop-infected cells. White arrowheads depict DAPI accumulation at the vAC and at the periphery. (B) Intracellular distribution of pp150 was categorized into three phenotypes: accumulations only at cell extensions (1), accumulations at cell extensions and at the vAC (2), and strict localization at the vAC (3). The nuclei were stained with DAPI and are visible in white. (C) Summary of the relative proportions of the three categories from three independent experiments normalized to 100%. For each individual experiment and virus, the intracellular distribution of pp150 was categorized from 25 infected cells that were positive for both pp150 and pUL48.