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Regulation of the E8 promoter by E1 and E2. RTS3b cells were cotransfected with 0.5 ng of pCMV-Gluc, the indicated reporter plasmids (50 ng), and the empty vector (−) or a combination of 100 ng pSG16 E1co and 10 ng pSG16 E2 (+).Values are presented as the ratios of fluc to gluc activities relative to 16URR (set as 1). Error bars indicate the SEM. Statistical significance was calculated with a one-way ANOVA and Tukey's multiple-comparison test (***, P < 0.001).
