FIG 7.

Analysis of HPV16 E8 promoter mutant genomes. (A) Southern blot analysis of low-molecular-weight DNA from two different sets of HPV16 wt (wt) and E8P mt2b/3b (mt) NHK cell lines. The linearized HPV16 genome (100 pg) was used as the molecular size marker (M). Viral copy number in the low-molecular-weight fraction was determined by qPCR and indicates the number of HPV16 E2 copies/2 beta-actin copies. (B) qPCR analysis of different viral transcripts. Values represent the number of viral transcript copies per cellular PGK1 copies.