Figure 1.

CD133 antigens used for mAb production. (A) Topological map of CD133 protein. Recombinant chimeric CD133 antigens, consisting of aa residues 171–420 and 507–716 (dotted line), were generated. (B) The two antigens, each tagged by an N-terminal 6xHis-tag, were expressed in E. coli and purified. The recombinant antigens were further verified by WB analysis with mouse anti-His mAb. Lane 1, ectodomain 1, Lane 2, ectodomain 2. CD133, prominin-1; mAb, monoclonal antibody; aa, amino acid; WB, Western blot.