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CD133 expression in glioblastoma U87 cells. (A) The cell lysates of U87, U87-CD133 overexpression and the control cell line Caco-2 were analyzed by WB analysis using C2E1 and the commercially-available antibody, C24B9. (B) Immunohistochemical staining with C2E1 revealed endogenous CD133 expression in the U87 cells. Pre-immunized serum served as a negative control. Scale bar, 20 µm. CD133, prominin-1; WB, western blot.
