Table II.
Quantitative polymerase chain reaction analysis of the relative expression levels of bone formation-associated genes.
| Gene | Day 1 | Day 4 | Day 7 | Day 14 |
|---|---|---|---|---|
| ALP | ||||
| pMSCV | 1 | 1.18±0.045 | 3.719±0.23 | 7.971±0.32 |
| pMSCV-DLX2 | 1.193±0.076 | 3.387±0.172 | 7.93±0.223 | 9.117±0.305 |
| OCN | ||||
| pMSCV | 1 | 1.084±0.058 | 3.79±0.271 | 5.56±0.217 |
| pMSCV-DLX2 | 1.013±0.075 | 1.057±0.087 | 3.873±0.215 | 6.482±0.035 |
| RUNX2 | ||||
| pMSCV | 1 | 3.104±0.083 | 2.86±0.395 | 3.626±0.217 |
| pMSCV-DLX2 | 1.081±0.104 | 3.209±0.149 | 3.02±0.308 | 3.481±0.03 |
| MSX2 | ||||
| pMSCV | 1 | 1.104±0.083 | 2.882±0.313 | 3.54±0.046 |
| pMSCV-DLX2 | 8.901±0.223 | 12.676±0.641 | 13.02±0.308 | 13.615±0.236 |
Statistically significant differences between the control and experimental groups were observed in the expression of ALP (P<0.01, on days 4, 7, 14), OCN,(P<0.05, on day 14) and MSX2 (P<0.01, on day 1, 4, 7, 14), but not in the expression of RUNX2 (P>0.05). DLX, distal-less gene; ALP, alkaline phosphatase; OCN, osteocalcin; RUNX2, runt-related transcription factor; MSX, Msh homeobox; MSCV, murine stem cell virus.