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. 2015 Jun 19;5:11410. doi: 10.1038/srep11410

Figure 2. Normal steady-state hematopoiesis in LPA4-deficient mice.

Figure 2

(A-F) Measurement of hematopoietic parameters in the BM. Total BM cellularity (A), HSPC number (B), HSC numbers defined as CD34− LSK and CD41−CD48−CD150+ LSK cells (D,E, respectively) and HPC number (F) in the BM (n = 9–11). Representative flow cytometry gating of CD41−CD48−CD150+ LSK HSC population is shown in (C). (G) Cell cycle status assessed through Ki-67 and DAPI staining. (H,I) Cell cycle status of HSPCs (H) and HSCs (I) in the BM (n = 9). (J and K) HSPC number (J) and HSC number (K) in the spleen (n = 8–9). (L) FACS analyses of donor-derived cells in PB three months after BM transplantation. (M) Analyses of BM chimeras of WT and LPA4-deficient mice reconstituted with Ly5.1 WT BM (n = 8–9). (N) Analyses of BM chimeras of Ly5.1 WT mice reconstituted with WT or LPA4-deficient BM (n = 7–8). *P < 0.05.