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. 2015 Apr 23;9(6):2910–2916. doi: 10.3892/ol.2015.3137

Figure 4.

Figure 4.

Overexpression of miR-134 inhibits VIM protein level in SKOV3-TR30 cells. (A) miR-134 expression in SKOV3-TR30 cells after transfection of miR-134 mimics was measured by qRT-PCR normalized to the expression of U6. The data are presented as the mean and SD of triplicate samples (*P﹤0.05, *P﹤0.01). The results were expressed as Log10 (2−ΔΔCT). (B) The analysis of the VIM mRNA level was performed in the above mentioned cells lines. Total RNA was extracted and subjected to real-time PCR to analyze the expression level of VIM. The data are presented as the mean and SD of triplicate samples. (C) Western blot analysis for endogenous VIM protein level using antibody against VIM in SKOV3 and SKOV3-TR30 cells and VIM in SKOV3-TRT30 cells transfected with miR-134 mimic and miRNA mimic negative control. GAPDH was used as internal control. Similar results were obtained in three independent experiments. VIM, vimentin; PCR, polymerase chain reaction; SD, standard deviation.