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(a) Cross-validated, Raman-based predictions of Tenofovir concentration in homogenized, porcine vaginal tissue versus gold-standard measurements acquired with LC-MS/MS. The root mean squared error of cross-validation (RMSECV) was 0.03% w/w for an integration time of 10 minutes per sample. (b) RMSECV versus total integration time in minutes (t) per sample. The solid lines represent a power-law fit and the 95% confidence interval of the fit.
