Fig. 3.

a) Reconstructed trajectory of a 1 μm fluorescent bead moved in a periodic 3D spiral pattern by the stepper-motor stage over a FOV of 15 μm (x-y) and 30 μm (z). Axial tracking is performed using the objective piezo-stage. b) Reconstructed trajectory of a 1 μm fluorescent bead moved in a periodic 3D spiral pattern by the stepper-motor stage over a FOV of 15 μm (x-y) and 30 μm (z). Axial tracking is performed using the ETL. For both experiments reported in a and b a 40x 0.8 NA water objective was used. c) Frequency distribution (log-scale) of the measured distance between scanners position and localized particle position for the test trajectories generated in a and b. d) Sedimentation profile of a 15 μm fluorescent bead over an axial range of 350 μm followed using the electrical lens and a 20x 0.4NA objective.