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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2015 Jul;74(7):710–722. doi: 10.1097/NEN.0000000000000210

Figure 5.

Figure 5

Cardiac glycosides suppress hypoxia inducible factor 1α (HIF-1α) expression in vitro and in vivo. (a) Western blot showing hypoxia induction of HIF-1α in MGG123 cells and its abrogation by digoxin [D] and ouabain [O] (1 μM). N, normoxia. Actin was used as a loading control. (b) Immunohistochemistry for HIF-1α on sections derived from an in vivo pharmacodynamic study testing systemic digoxin treatment. Upper panels show results obtained in 5 mice in the vehicle group (V1–V5); bottom panels show matching results from 5 mice in the digoxin group (D1–D5). B, brain; T, tumor. Scale bars: 100 μm. (c) Table summarizing the HIF-1α immunopositivity status in all the animals.