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. 2015 Jun 20;8:252. doi: 10.1186/s13104-015-1222-9

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© Benner et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Visualization of neurons under a fluorescence radiated field, of a mouse hippocampus stained with NeuroTrace a without fixation and b with ethanol fixation. c A single dentate gyrus (DG) was collected, d 150,000 μm² × 20 μm in volume, for the following each experimental condition: unfixed and unstained, NeuroTrace stained without ethanol fixation, ethanol fixation without staining, and ethanol (EtOH) and NeuroTrace (NT) treated. Transcript levels of e 18S rRNA and f GAPDH mRNA. Values are expressed as copy number of transcripts. Bars indicate mean ± SEM (n = 6 each). One-way ANOVA followed by Tukey post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars 310 μm.