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. 2015 Jun 20;8:252. doi: 10.1186/s13104-015-1222-9

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© Benner et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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a H & E-stained pituitary cryosection on a silane-coated microslide. b Ethanol-fixed and NeuroTrace-stained pituitary sections under (b ₁) bright field and (b ₂, b ₃) fluorescence radiated field. c–e Comparative analysis between the anterior and posterior pituitary showing specific transcript expression profile for each region. c corticotropin-releasing hormone receptor 1; CRHR1, d vasopressin; AVP, and e oxytocin; OXT. The transcript levels in the posterior pituitary values (n = 8) were expressed as fold change compared to the anterior pituitary values (n = 9) and after normalization to GAPDH. Bars indicate mean ± SEM. Asterisks (***p < 0.001) indicate statistically significant difference between samples collected from the anterior and posterior pituitary glands, as assessed by Student’s t-test. Scale bars a, b 310 μm, and c 100 μm.