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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Gene. 2015 May 7;568(1):19–24. doi: 10.1016/j.gene.2015.05.012

FIG 2.

FIG 2

Analysis of mDab2ip methylation during cerebellar development at postnatal days P8, P14, P21 and P30. (A) Methylation specific PCR of CpG131, CpG54, and CpG85 using PCR primers which specifically amplified methylated (M) or unmethylated (U) CpG islands. (B) Bisulfite sequencing of mDab2ip CpG131, CpG54, and CpG85 from cerebella (n=3) collected at indicated postnatal days. (C) Quantitation of CpG131, CpG54, and CpG85 methylation during cerebellar development by bisulfite sequencing. Methylation is shown as an average percentage of total CpG’s which were found to be methylated in three separate animals at each time point. Statistical analysis was performed using a two-tailed Student’s t-test (unequal variance). *, p<0.05, **, p < 0.01.