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. 2015 Apr 28;66(13):3753–3764. doi: 10.1093/jxb/erv168

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — (A) Longitudinal sections of the different phases of Brachypodium distachyon germination sensu stricto, stained with toluidine blue. C, coleorhiza; Co, coleoptile; E, endosperm; Sc, scutellum; Sh, shoot; R, root. Scale bar, 200 μm. (B) Endo-β-mannanase activity (white bars) in embryo and endosperm (de-embryonated seed) upon B. distachyon seed germination (0–24h). One unit of MAN activity is defined as the amount of enzyme that releases 1 nmol of reducing sugar per minute and per mg of protein. Percentage germination evaluated as coleorhiza emergence (CE; open circles) and root emergence (RE; close circles) are represented. In the inset, the time needed for 50% of CE (t₅₀CE) and RE (t₅₀RE) is indicated. Data are means ± standard error (SE) of three technical replicates of three biological samples.