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. 2015 Apr 28;66(13):3753–3764. doi: 10.1093/jxb/erv168

Fig. 6.

Fig. 6.

In situ mRNA hybridization analysis of BdMAN2, BdMAN4 and BdMAN6 in 27h germinating Brachypodium seeds. (A–D) BdMAN2, (E–H) BdMAN4, (I–L) BdMAN6. (A, E, I) Longitudinal sections of germinating embryos. (B, F, J) Close-up of the coleorhiza and the root tip. (C, G, K) Close-up of the endosperm and the aleurone. (D, H, L) Control sense probes. Al, aleurone layer; C, coleorhiza; Co, coleoptile; Cp, calyptra; E, endosperm; Sc, scutellum; Sh, shoot; R, root. The black arrow indicates the localization of transcripts. Scale bar, 50 μm.