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. 2015 Apr 21;66(13):3815–3829. doi: 10.1093/jxb/erv185

Fig. 5.

Fig. 5.

Analyses of cytosolic Cd in AtABCC3ox leaf protoplasts and of Cd content in AtABCC3ox shoots, roots, and seedlings. Protoplasts from AtABCC3ox-21 and AtABCC3ox-26 overexpressing plants grown in the absence or presence of 60 μM CdSO4, with or without the inducer β-oestradiol, were loaded with the cytosolic Cd-sensing fluorochrome BTC-5N. (A–F) Fluorescent images of protoplasts loaded with BTC-5N. AtABCC3ox-21 (A) and AtABCC3ox-26 (D) protoplasts from plants grown in the absence of Cd for 9 d. AtABCC3ox-21 (B, C) and AtABCC3ox-26 (E, F) protoplasts from plants grown in the presence of 60 μM CdSO4 with (C, F) or without (B, E) β-oestradiol for 9 d compared with those from plants grown without β-oestradiol. Cytosolic signal is indicated by arrows. (G) Fluorescence signal intensity in the cytosol of AtABCC3ox-21 and AtABCC3ox-26 protoplasts from plants grown in the absence or presence of Cd with or without β-oestradiol. Values are means (n=30). Error bars indicate the SE. Asterisks indicate a significant difference from the absence of β-oestradiol within the same genotype (**P<0.01). (H–J) Cd content in roots (H), shoots (I), and seedlings (J) of AtABCC3ox-21 and AtABCC3ox-26 seedlings overexpressing AtABCC3 compared with wild type seedlings. Error bars indicate the SE (n=3). est, β-oestradiol. Scale bars=10 μm.