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. 2015 Apr 21;66(13):3815–3829. doi: 10.1093/jxb/erv185

Fig. 8.

Fig. 8.

Comparative analysis of AtABCC3, AtABCC2, and AtABCC1 transcript levels in wild type, abcc3, and atabcc1 atabcc2 seedlings exposed to 60 μM CdSO4 and in wild type seedlings at different Cd concentrations. (A) Real-time RT–PCR of mRNA extracted from wild type, abcc3, and abcc1abcc2 seedlings grown for 9 d at 0 or 60 μM CdSO4 (as indicated). Data are expressed as a mean value (n=3) of AtABCC3, AtABCC1, and AtABCC2 cDNA levels relative to actin cDNA. Error bars indicate the SE. (B) Real-time RT–PCR of mRNA extracted from wild type seedlings grown for 9 d at 0, 15, 30, and 60 μM CdSO4. Data are expressed as a mean value (n=3) of AtABCC3 AtABCC1, and AtABCC2 cDNA levels relative to actin cDNA. Error bars indicate the SE. Asterisks indicate a significant difference in AtABCC3 transcript level from wild type seedlings at 0 μM CdSO4 (**P<0.01, ***P<0.001). Dots indicate a significant difference from AtABCC3 transcript level in wild type seedlings at 60 μM CdSO4 (••P <0.01). Circles indicate a significant difference in AtABCC1 and AtABCC2 transcripts from the AtABCC3 transcript level in wild type seedlings grown in the absence of Cd (°P< 0.05, °°°P< 0.001). wt, wild type.