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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Electrophoresis. 2014 Jun 5;35(0):1855–1863. doi: 10.1002/elps.201400040

Table 2.

Acronyms, sequences, and properties of linker modified DNA oligomers.

Acronym1 DNA Sequence2 Net Charge Mobility, m.u.3 Mobility Ratio4
ss20/1 GCCTATGAAAGGTCTCGACC −17 3.115 0.966
ss20/4 TTTGTGTCCCCCTCTCAGGT −11 2.59 0.804
ss20/6 CACAAACCTGTTCTTGGCAG −7 1.58 0.490
ss20/6 TCGGACTCTGAGTTTCGGTT −7 1.64 0.509
ss20/0 GCCTATGAAAGGTCTCGACC −19 3.22 1.000
ss23/1 TTGGCCTATGAAAGGTCTCTCTAAT −20 3.07 0.948
ss23/0 TTGGCCTATGAAAGGTCTCTCTAAT −22 3.24 1.000
ds20/1 GCCTATGAAAGGTCTCGACCCGGATACTTTCCAGAGCTGG −36 3.24 0.953
ds20/0 GCCTATGAAAGGTCTCGACCCGGATACTTTCCAGAGCTGG −38 3.40 1.000
ds23/1 TTGGCCTATGAAAGGTCTCTCTAATAACCGGATACTTTCCAGAGAGATTA −42 3.28 0.962
ds23/0 TTGGCCTATGAAAGGTCTCTCTAATAACCGGATACTTTCCAGAGAGATTA −44 3.41 1.000
1

The acronyms indicate whether the oligomer is single- or double-stranded, the number of nucleotides or base pairs, followed by a slash and the number of positively charged internucleoside DEED linkers. Controls without DEED linkers have a zero following the slash.

2

The nucleotides in bold precede a cationic DEED linker.

3

Measured in 20 mM HEPES buffer or 20 mM KDM buffer; the mobilities in these two buffers were equal within experimental error. The average standard deviation of the mobilities was ±0.03 m.u.

4

Mobility ratio = mobility (charge variant) / mobility (parent DNA with the same number of nucleotides or base pairs).

5

Average mobility of a closely spaced doublet in the electropherogram.