Fig. 1.

(A). Human lenses revealed age-dependent increases of Sumo1 conjugates. Total proteins were isolated from freshly obtained eye lenses and equal amounts of protein were loaded onto 4-20% SDS PAGE gel. Sumo1 conjugates and free Sumo1 were visualized by anti-Sumo1 antibody. (B). Prdx6-deficient mLECs bore increased Sumo1 conjugates and elevated expression of ROS. Total cell lysates were prepared from Prdx6^+/+ and Prdx6^−/− cells and immunoblotted with Sumo1 antibody (a), and quantification of ROS levels (b) Prdx6^−/− with H2-DCF-DA showed involvement of oxidative stress (b). Histogram values are mean ± SD from three independent experiments. O.D., optical density.* Statistically significant difference (p<0.001 vs control).