FIG 6.

p23 is at least partially responsible for ER rearrangement during BBSV infection. Agroinfiltrated leaf tissues were embedded, ultrasectioned, and observed under TEM. (A and B) Transient expression of p23 alone in N. benthamiana leaf tissues induces convolution and aggregation of the ER. (C) Electron-dense structures were also frequently observed in leaf tissues agroinfiltrated with p23. (D) Higher magnification of the area enclosed by the dashed line in panel C. The electron-dense areas are connected to the ER (arrowheads). (E) There was no obvious abnormality in the ER structure in leaves agroinfiltrated with empty vector. The expression of p23 in the agroinfiltrated leaves was confirmed by Western blotting (see Fig. S3C in the supplemental material, 35S-p23). Chl, chloroplast; Mit, mitochondria; CW, cell wall. Scale bars = 200 nm.