FIG 7.

Exogenous addition of gangliosides does not alter JCPyV infection. (A) Permissive SVGA cells in a 24-well plate were supplemented with a 30 μM concentration of the indicated gangliosides or DMSO (vehicle control) overnight and then washed with medium and infected with JCPyV Mad-1, Mad-1/WT3C, or BKPyV and SV40 as controls. Infected cells were scored by indirect immunofluorescence for nuclear VP1 at 72 h. (B) Poorly permissive HEK293A cells were supplemented and infected as for panel A, and infected cells were scored by indirect immunofluorescence for nuclear T antigen at 48 h postinfection. Data represent the average number of infected cells per visual field for five 10× fields of view for triplicate samples. Error bars indicate SD. (C) SVGA cells were supplemented as for panel A and then infected with Mad-1, Mad-1/WT3C, or BK pseudovirus (control). Supernatant from infected cells was collected and analyzed for secreted luciferase. The average relative luciferase units (RLU) for triplicate samples are shown in log scale. Error bars represent SD. These data are representative of 3 experiments.