FIG 2.

Virus sensitivity to 5-FU treatment. DBT-9 cells were pretreated with the indicated concentration of 5-FU and infected with the indicated virus at an MOI of 1 PFU/cell (A and C) or 0.01 PFU/cell (B, D, and E). Virus supernatants were harvested at 12 h postinfection (MOI = 1) or 24 h postinfection (MOI = 0.01), and the virus titer was determined by a plaque assay. For each virus, titers were normalized to values obtained from DMSO-treated samples using Prism 6. Data are presented as means ± standard errors of the means (SEM). Statistical significance compared to WT-ExoN(+) or MHV-ExoN(−) (panel E) is denoted (*, P < 0.05; **, P < 0.01; ***, P < 0.0001) and was determined using one-way analysis of variance (ANOVA) with Dunnett's post hoc test for multiple comparisons (n = 4 to 7).