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5-FU sensitivity of R80A/E82A-ExoN(−) compared to MHV-ExoN(−). DBT-9 cells were pretreated with the indicated concentration of 5-FU and infected at an MOI of 0.01 PFU/cell. Supernatants were harvested at 24 h postinfection, and the virus titer was determined by a plaque assay. Data are presented as means ± SEM. There was no statistically significant difference between R80A/E82A-ExoN(−) and MHV-ExoN(−) at each concentration as determined by a two-tailed unpaired t test (n = 4).
