FIG 7.

Virus-induced mitochondrial apoptosis in LCMV-pi cells is RIG-I independent but requires MAVS. (A and B) LCMV-pi A549 cells were depleted of RIG-I and MAVS using specific siRNAs, and efficiency of knockdown was verified by Western blotting using tubulin as a loading control. (C and D) Persistent LCMV prevents induction of IFN-β via RIG-I and MAVS. LCMV-pi and mock-infected control cells depleted of RIG-I or MAVS were superinfected with VSV (MOI of 3) for 16 h. The induction of IFN-β mRNA was assessed by RT-qPCR as described in the legend of Fig. 3B. Data represent fold induction above the level seen in uninfected (u) and control siRNA (C)-treated cells (n = 3; means ± standard deviations). (E and F) Virus-induced apoptosis in LCMV-pi cells is independent of RIG-I but requires MAVS. Cells were depleted of RIG-I and MAVS and then superinfected with VSV as described for panels C and D. PARP cleavage was detected in cell lysates by Western blotting as described in the legend of Fig. 1D, using tubulin as a loading control.