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Virus-induced mitochondrial apoptosis in LCMV-pi cells is independent of MDA5 and NOD2. LCMV-pi A549 cells were depleted of MDA5 (A) and NOD2 (C) by transfection of specific siRNAs and control siRNAs. Efficiency of knockdown was verified by RT-qPCR. LCMV-pi and mock-infected control cells depleted of MDA5 (B) and NOD2 (D) were superinfected with VSV (MOI of 3). After 16 h, cells were lysed, and PARP cleavage was detected by Western blotting using tubulin as a loading control.
