TABLE 3.
Revertant descriptions
| ts mutation | Revertant | Substitution(s) identified in the revertant | Nucleotide sequencea |
|---|---|---|---|
| PA T210P | ts210/Rev1 | PA P210T | GGA ACA ATG/GGA ACA ATG |
| ts210/Rev2 | PA E377K | CCA AAA AAG/CCA GAA AAG | |
| ts210/Rev3 | PA A432V + PB1 R287M | GAT GTG GCT/GAT GCG GCT | |
| GTA ATG AAG/GTA AGG AAG | |||
| ts210/Rev4 | PA A20T | AAG ACA ATG/AAG GCA ATG | |
| ts210/Rev5 | PB2 R136G + PB2 D309N | TTT GGA AAC/TTT AGA AAC | |
| GTG AAT ATT/GTG GAT ATT | |||
| PA D216P | ts210/Rev6 | PB1 R287M | GTA ATG AAG/GTA AGG AAG |
| ts216/Rev1 | PA P216D | GCC GAC CAA/GCC GAC CAA | |
| ts216/Rev2 | PA P216S | GCC TCC CAA/GCC GAC CAA | |
| PA L219P | ts219/Rev1 | PA P219L | AGT CTT CCG/AGT CTC CCG |
| ts219/Rev2 | PA P219L + PA A20T | AGT CTT CCG/AGT CTC CCG | |
| AAG ACA ATG/AAG GCA ATG | |||
| PA F223P | ts223/Rev1 | PA P223H | AAC CAC TCC/AAC TTC TCC |
a
Nucleotide sequences for the revertant and wild-type sequences (the two sequence types are divided by a slash; the mutated codons are surrounded by −1 and +1 codons).