FIG 3.

JEV E protein structure (PDB accession no. 3P54) with the location of amino acid differences between JEV SA14-14-2 and wild-type rJEV India/78 indicated. Amino acid substitutions are located in structural domain I (residues 138, 176, 177, and 279), domain II (residues 107, 244, and 264), and domain III (residues 315 and 366). Residue 439 is located in the stem-anchor portion of the E protein and is not pictured here. Boxed residues indicate the locations of amino acids that consistently differed between vaccine virus and parent virus sequences and were considered vaccine strain specific.