TABLE 4.
Virulence screening of rJEV mutants and control viruses in weanling mice
Virus | i.p. inoculation |
i.c. inoculation |
||||||||
---|---|---|---|---|---|---|---|---|---|---|
Dose (PFU) | No. of alive mice/total no. of mice | % survival | Median survival time (days) | Significant difference from rJEVa | Dose (PFU) | No. of alive mice/total no. of mice | % survival | Median survival time (days) | Significant difference from rJEVa | |
rJEVb | 104 | 1/20 | 5 | 7.5 | NA | 102 | 0/20 | 0 | 5.5 | NA |
rJEVmut1 | 104 | 7/10 | 70 | >21 | Yes | 102 | 1/10 | 10 | 5.5 | No |
rJEVmut2 | 104 | 7/10 | 70 | >21 | Yes | 102 | 0/10 | 0 | 6 | Yes |
rJEVmut3 | 104 | 9/10 | 90 | >21 | Yes | 102 | 6/9c | 67 | >21 | Yes |
rJEVmut4 | 104 | 3/10 | 30 | 9 | No | 102 | 0/10 | 0 | 5 | No |
rJEVmut5/6 | 104 | 4/10 | 40 | 7.5 | No | 102 | 0/10 | 0 | 5 | No |
rJEVmut7 | 104 | 4/10 | 40 | 6 | No | 102 | 1/10 | 10 | 5 | No |
rJEVmut8 | 104 | 2/10 | 20 | 7 | No | 102 | 0/10 | 0 | 6 | Yes |
rJEVmut9 | 104 | 1/10 | 10 | 6 | No | 102 | 0/10 | 0 | 5 | No |
rJEVmut10 | 104 | 4/10 | 40 | 12.5 | Yes | 102 | 0/10 | 0 | 5 | No |
rJEVmut11 | 104 | 10/10 | 100 | >21 | Yes | 102 | 8/10 | 80 | >21 | Yes |
rJEVmutE | 104 | 10/10 | 100 | >21 | Yes | 102 | 10/10 | 100 | >21 | Yes |
rJEVmutNS1/2A | 104 | 7/10 | 70 | >21 | Yes | 102 | 0/10 | 0 | 6 | No |
rJEVmutNS5 | 104 | 1/10 | 10 | 7 | No | 102 | 0/10 | 0 | 5 | No |
rJEVmut14-14-2 | 104 | 10/10 | 100 | >21 | Yes | 104 | 10/10 | 100 | >21 | Yes |
JEV SA14-14-2 | 104 | 10/10 | 100 | >21 | Yes | 104 | 10/10 | 100 | >21 | Yes |
Mockb | NAd | 20/20 | 100 | >21 | Yes | NA | 20/20 | 100 | >21 | Yes |
Determined by the log rank test, with a P value of <0.05 being required for significance.
Results from two different experiments were combined.
One mouse died from trauma at the injection site.
NA, not applicable.