Skip to main content
. 2015 Apr 8;89(12):6328–6337. doi: 10.1128/JVI.00219-15

TABLE 4.

Virulence screening of rJEV mutants and control viruses in weanling mice

Virus i.p. inoculation
i.c. inoculation
Dose (PFU) No. of alive mice/total no. of mice % survival Median survival time (days) Significant difference from rJEVa Dose (PFU) No. of alive mice/total no. of mice % survival Median survival time (days) Significant difference from rJEVa
rJEVb 104 1/20 5 7.5 NA 102 0/20 0 5.5 NA
rJEVmut1 104 7/10 70 >21 Yes 102 1/10 10 5.5 No
rJEVmut2 104 7/10 70 >21 Yes 102 0/10 0 6 Yes
rJEVmut3 104 9/10 90 >21 Yes 102 6/9c 67 >21 Yes
rJEVmut4 104 3/10 30 9 No 102 0/10 0 5 No
rJEVmut5/6 104 4/10 40 7.5 No 102 0/10 0 5 No
rJEVmut7 104 4/10 40 6 No 102 1/10 10 5 No
rJEVmut8 104 2/10 20 7 No 102 0/10 0 6 Yes
rJEVmut9 104 1/10 10 6 No 102 0/10 0 5 No
rJEVmut10 104 4/10 40 12.5 Yes 102 0/10 0 5 No
rJEVmut11 104 10/10 100 >21 Yes 102 8/10 80 >21 Yes
rJEVmutE 104 10/10 100 >21 Yes 102 10/10 100 >21 Yes
rJEVmutNS1/2A 104 7/10 70 >21 Yes 102 0/10 0 6 No
rJEVmutNS5 104 1/10 10 7 No 102 0/10 0 5 No
rJEVmut14-14-2 104 10/10 100 >21 Yes 104 10/10 100 >21 Yes
JEV SA14-14-2 104 10/10 100 >21 Yes 104 10/10 100 >21 Yes
Mockb NAd 20/20 100 >21 Yes NA 20/20 100 >21 Yes
a

Determined by the log rank test, with a P value of <0.05 being required for significance.

b

Results from two different experiments were combined.

c

One mouse died from trauma at the injection site.

d

NA, not applicable.