TABLE 4.
Virulence screening of rJEV mutants and control viruses in weanling mice
| Virus | i.p. inoculation |
i.c. inoculation |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dose (PFU) | No. of alive mice/total no. of mice | % survival | Median survival time (days) | Significant difference from rJEVa | Dose (PFU) | No. of alive mice/total no. of mice | % survival | Median survival time (days) | Significant difference from rJEVa | |
| rJEVb | 104 | 1/20 | 5 | 7.5 | NA | 102 | 0/20 | 0 | 5.5 | NA |
| rJEVmut1 | 104 | 7/10 | 70 | >21 | Yes | 102 | 1/10 | 10 | 5.5 | No |
| rJEVmut2 | 104 | 7/10 | 70 | >21 | Yes | 102 | 0/10 | 0 | 6 | Yes |
| rJEVmut3 | 104 | 9/10 | 90 | >21 | Yes | 102 | 6/9c | 67 | >21 | Yes |
| rJEVmut4 | 104 | 3/10 | 30 | 9 | No | 102 | 0/10 | 0 | 5 | No |
| rJEVmut5/6 | 104 | 4/10 | 40 | 7.5 | No | 102 | 0/10 | 0 | 5 | No |
| rJEVmut7 | 104 | 4/10 | 40 | 6 | No | 102 | 1/10 | 10 | 5 | No |
| rJEVmut8 | 104 | 2/10 | 20 | 7 | No | 102 | 0/10 | 0 | 6 | Yes |
| rJEVmut9 | 104 | 1/10 | 10 | 6 | No | 102 | 0/10 | 0 | 5 | No |
| rJEVmut10 | 104 | 4/10 | 40 | 12.5 | Yes | 102 | 0/10 | 0 | 5 | No |
| rJEVmut11 | 104 | 10/10 | 100 | >21 | Yes | 102 | 8/10 | 80 | >21 | Yes |
| rJEVmutE | 104 | 10/10 | 100 | >21 | Yes | 102 | 10/10 | 100 | >21 | Yes |
| rJEVmutNS1/2A | 104 | 7/10 | 70 | >21 | Yes | 102 | 0/10 | 0 | 6 | No |
| rJEVmutNS5 | 104 | 1/10 | 10 | 7 | No | 102 | 0/10 | 0 | 5 | No |
| rJEVmut14-14-2 | 104 | 10/10 | 100 | >21 | Yes | 104 | 10/10 | 100 | >21 | Yes |
| JEV SA14-14-2 | 104 | 10/10 | 100 | >21 | Yes | 104 | 10/10 | 100 | >21 | Yes |
| Mockb | NAd | 20/20 | 100 | >21 | Yes | NA | 20/20 | 100 | >21 | Yes |
a
Determined by the log rank test, with a P value of <0.05 being required for significance.
b
Results from two different experiments were combined.
c
One mouse died from trauma at the injection site.
d
NA, not applicable.