FIG 7.

The DNA replication stimulatory effect of PUBS is antagonized by catalytically inactive USPs. (A to C) Levels of HPV DNA replication in cells expressing the indicated E1 proteins and increasing amounts of RFP-tagged USP1ci (A), USP12ci (B), or USP46ci (C). Cells cotransfected with the empty RFP vector were used as a negative control (−). DNA replication activity is reported as a percentage of the signal obtained with the negative control, which was set at 100%. Assays performed in the absence of E1 were used as baseline (i.e., no DNA replication) controls (×). Each value represents the average from three independent experiments, each performed in triplicates, with the standard deviation indicated by an error bar.