FIG 3.

SRSF1 inhibition of replication in divergent viral subtypes. (A) Primary sequences alignments of the characterized SRSF1 binding sites in molecular clones from the B (NL4.3 and LAI.2), C (MJ4), and D (94UG114.1.6) viral subtypes. (B to E) Quantification of viral replication and viral mRNAs for the four viral molecular clones after expression of the SRSF1 constructs. Replication for each viral clone was assayed as described in Fig. 2C. The relative amount of viral RNA was quantified as described in Fig. 2D. The relative amounts of gag/pol and multiple spliced mRNAs were quantified as described in Fig. 2E, normalized for the total viral mRNA content of each sample, and expressed as the fold increase or decrease versus the control pEGFP transfection. All data are represented as means ± the SEM.