Figure 2. CdGAP over expression slows random cell migration.

(A) U2OS cells were transfected with GFP or GFP-cdGAP. (B) Representative tracks of transfected U2OS cells plated at low density migrating on 10µg/ml collagen for 16 hours, with GFP-cdGAP transfected cells migrating a shorter distance than GFP control cells. (C) GFP expressing cells migrate at a higher velocity than GFP-cdGAP expressing cells. A minimum of 30 cells from three independent experiments were analyzed per condition and GFP versus GFP-cdGAP expressing cells compared with Student’s t-test,***, p < 0.001. Velocity graph has error bars representing +/− the standard error of the mean.