Table 4.

Estimated effects of factors, F-ratios, and associated P-values for spreadability (%) and ACV permeated (%)

Factor	Y1			Y2
	Estimate	F-ratio	P-values	Estimate	F-ratio	P-values
X1	−0.4	12.63	0.0163*	−8.5	5.67	0.0631
X2	−0.5	19.74	0.0067*	−17.25	23.35	0.0047*
X3	−0.1	0.79	0.4150	11.75	10.84	0.0217*
X12	−0.483	8.51	0.0331*	−12.583	5.74	0.0620
X1X2	0.4	6.32	0.0536	21.0	17.31	0.0088*
X1X3	0.0	0.00	1.0000	−3.0	0.35	0.5782
X22	0.417	6.33	0.0535	3.917	0.56	0.4895
X2X3	−0.1	0.39	0.5574	−0.5	0.01	0.9249
X32	0.517	9.73	0.0263*	17.917	11.63	0.0190*

Notes:

^*Significant effect of factors on individual responses. X₁, molecular weight of chitosan; X₂, percentage of chitosan; X₃, percentage of Eugenol; Y₁, spreadability (%); Y₂, percent of ACV permeated (%). X₁X₂, X₁X₃, and X₂X₃ are the interaction terms between the factors.

Abbreviation: ACV, acyclovir.