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. 2015 Jun 15;9:3051–3066. doi: 10.2147/DDDT.S82146

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© 2015 Chen et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Ex vivo characterization of changes in oxidative stress in rats upon I/R-induced injury.

Notes: Quantitative assessment of the antioxidative stress effect of QSYQ was done based on SOD (A), MDA (B) of rat plasma using an ELISA kit following the manufacturer’s instructions (Ref), and the ROS (C) in myocardial mitochondrial using DCFH-DA fluorescent probes detection kit as previously described (Ref). Data are expressed as mean ± SD (each group, n=8). **P<0.01 compared with the Sham group; ^##P<0.01 compared with the I/R group.

Abbreviations: I/R, ischemia/reperfusion; QSYQ, QiShenYiQi Pill^®; SOD, superoxide dismutase; MDA, malondialdehyde; ROS, reactive oxygen species; DCFH-DA, 2′,7′-dichlorofluorescin diacetate; SD, standard deviation; ELISA, enzyme linked immunosorbent assay; FI, fluorescence intensity.